I'm fascinated by these brain proteomics papers. How much functioning in the brain can we detect by shifts in post translational modifications? It sure seems like a lot!
In this paper (in press at MCP and temporarily open access) from Karaki and Becamel et al., these researchers describe an approach to studying the global phosphorylation events that occur during exposure to active hallucinogens. They do this by using an extremely similar compound that can occupy the same binding sites, but does not actually induce hallucinations.
Using an Orbi Velos, they identify nearly 6,000 phosphorylation events and several that only occur when the active hallucinogen is given. They follow this up with an incredibly thorough study involving knock downs, immunocytochemistry and giving mice LSD. The number of experiments in this paper is pretty staggering. Above and beyond what we normally see in MCP. Not to put down MCP or nuthin, but if you are going to follow up your phosphoproteomics with this much validation and work you deserve to put this into Cell. It makes the rest of us look like slackers, quite honestly.
1) Great phosphoproteomics work
2) Amazing level of validation
3) Cool brain stuff
4) Mice on LSD (actually, it was DOI, similar though)
Why are you still reading this and not this great paper? Oh yeah. Direct link is here.
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